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dapi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dapi
    Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+d1306/DAPI+(4'%2C6-diamidino-2-phenylindole%2C+dihydrochloride)/pmc13634069-96-2-5
    Average 99 stars, based on 1 article reviews
    dapi - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    CRISPR:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Western Blot:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    MTT Assay:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Control:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Standard Deviation:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Comparison:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Immunofluorescence:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Software:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Immunostaining:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Gene Expression:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Infection:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Fluorescence:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Quantitative RT-PCR:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Expressing:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Plaque Assay:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Epifluorescence Microscopy:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    In Situ Hybridization:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    SYBR Green Assay:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Incubation:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Microscopy:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Marker:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Confocal Microscopy:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Molecular Weight:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Synthesized:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Imaging:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).

    Lysis:

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: Then, the sections were counterstained with DAPI (D1306, Thermo, 1:1000) for 20 min.

    Article Title: Naringenin and SMER28 target lysosomal reformation and rescue SPG11 and SPG15 hereditary spastic paraplegia phenotypes.
    Article Snippet: Larvae were incubated with anti-Ref2P (1:200, ab178440, Abcam) O/N at 4◦C and successively with secondary CyTM5 Ab (1:500, Jackson ImmunoResearch Labs Cat# 111–175–144) and DAPI (D1306, Thermo Fisher Scientific) for 1 h a RT.

    Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking
    Article Snippet: The retentate was recovered (200-500μL) and protein concentration determined by BCA assay (Thermo Fisher, 23227).

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion
    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Article Title: Fatty Acid Synthase binds endogenous dsRNAs and dampens the innate immune response to exogenous dsRNAs by limiting their accumulation
    Article Snippet: DAPI (D1306, Invitrogen, Thermo Fisher Scientific) staining was performed for 5 minutes to reveal the nuclei.

    Article Title: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
    Article Snippet: 450 μL cells were fixed in 3.7% formaldehyde at 4 °C for 16 h. After washing with PBS, the cells were treated with PBS containing 0.5 mg/mL RNase A at 37 °C for 10 h. Then, the cells were stained with 2.5 ng/μL DAPI (Thermo-Fisher, D1306) dissolved in PBS at 25 °C for 4 h. The cells were washed with PBS and resuspended in 20 μL PBS.

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: Cells were subsequently incubated for 1 hour with fluorescence-labeled secondary antibodies and counterstained with DAPI (DAPI; Cat No. D1306; Thermo Fisher Scientific, Waltham, MA, USA).



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